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The Guava® Express 7-AAD reagent is a cell impermeant nulcear dye used in viability assays. 7-Aminoactinomycin-D (7-AAD) is a fluorescent DNA intercalator that is membrane impermeant (and therefore binds to dead cells). The 7-AAD dye included in the kit monitors cell membrane permeability changes typically ob served later in apoptosis, as well as necrotic cell death. 7-AAD is excluded from live, healthy cells as well as early apoptotic cells.
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文献和实验A SENSITIVE METHOD FOR DETECTION OF APOPTOSIS BY SINGLE LASER FLOW CYTOMETRY
MATERIALS : 1. 1 X PBS (PBSAz, 1 X PBS, e.g., Irvine Scientific, CA, containing 2% newborn calf serum and 0.1% sodium azide) 2. 7-Amino-actinomycin D (7-AAD, e.g., Calbiochem, CA) 3. Human AB serum, heat-inactivated
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