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- 详细信息
- 文献和实验
- 技术资料
- 库存:
27
- 英文名:
Cyanidin 3-O-β-D-Galactopyranoside (chloride)
- CAS号:
27661-36-5
- 供应商:
上海莼试
- 保存条件:
4°C, protect from light
- 规格:
1 mg 5 mg
分子式:C21H21O11Cl
分子量:484.8
溶解度:Methanol: slightly soluble,Water: slightly soluble
储存条件:4°C, protect from light
General tips:For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.
Shipping Condition:Evaluation sample solution : ship with blue ice All other available size: ship with RT , or blue ice upon request
Cyanidin 3-O-β-D-galactopyranoside is an anthocyanin flavonoid pigment that has been found in P. vera and has antioxidant properties. It scavenges radicals in 2,2-diphenyl-1-picrylhyrazyl , Trolox equivalent antioxidant capacity (TEAC), superoxide anion, and hydrogen peroxide assays. Cyanidin 3-O-β-D-galactopyranoside decreases the release of lactate dehydrogenase (LDH) and the activation of caspase-3 in lymphocytes with t-butyl hydroperoxide-induced oxidative damage.
商品属性:
| 货号 | CS-01Y76082 | 规格 | 1 mg 5 mg |
| CAS号 | 27661-36-5 | 分子量 | 484.8 |
| 含量 | >99.00% | 别名 | N/A |
| 分子式 | C21H21O11Cl | 化学名 | N/A |
| 产地 | 国产 | 用途 | 仅供科研研究实验 |
抗逆滴加序列
每次向板内滴加抗原时,移液器滴头要与平面45度悬空,不要触碰到孔内的液体,由后向前依次滴加(即浓度由低往高滴加)。
抗感染反应期
抗原抗体在室温20~25℃下,必须反应30min以上,若环境温度低于室温,可将微量反应板置于恒温培养箱中,使二者充分反应。
旅游温度
磷酸盐缓冲液的pH值要在高压灭菌后进行滴定,往往在高压后pH值会有所改变,所以高压后再调一次pH值更为准确。磷酸盐缓冲液一经使用保存期不要超过3周。当pH<5.8时,红细胞会产生自凝现象;当pH>7.8时,图形洗脱加快,易造成肉眼观察产生误差;p H=7.2时,红细胞沉降最充分,图形最清晰。
当滴注 1%红细胞悬液时,应经常摇晃红细胞悬液,使红细胞均匀地分布在磷酸缓冲液中,以防止红细胞下降。
反应时间及温度
加入鸡红血球之后,反应板在室温(20~25℃)静置30~40min,对照孔血球下沉于孔底,即可判定结果。若室温达不到实验要求,需相应调整反应时间。当环境温度低于4℃时,红细胞发生自凝;高于37℃时,会发生反应物分离和红细胞溶血。
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1.本蛋白酶抑制剂混合物为100×的储存液,使用时按照1:100的比例加入到裂解液中(例如,1ml裂解液中加入10μl蛋白酶抑制剂混合物),混匀后即可使用。根据需要,0.5M的EDTA也按照1:100的比例加入到裂解液中(如用于检测金属蛋白酶活性,则不宜添加EDTA)。含有蛋白酶抑制剂混合物的裂解液宜现用现配,不宜配制后冻存待后续使用。
2. 待所需的抑制剂添加完毕混匀后,就可以开始进行哺乳动物组织的裂解和蛋白提取。
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文献和实验Measurement of Chloride Movement in Neuronal Preparations
(GABA) or other agonist in recipeRingers solution Plexiglas imaging chamber (Fig. ) with outflow and inflow tubes (∼1.7‐mm‐o.d.; ∼1.2‐mm i.d.), flow rate restrictor (in line
reached 1.0, isopropyl-1-thio-β-d-galactopyranoside was added to a final concentration of 1 mM. After 5 h of induction, the cells were harvested by centrifugation at 4,000 × g for 20 min. 样品处理 For the purification of protein from 2 liters of culture
Preparation of Competent Cells (from PMB)
1.Inoculate a couple of 20 ml LB broth in a 125 ml flasks with 0.5 ml of an overnight culture of the recipient strain. 2.Shake at 37 ℃ until O.D. 600=0.13 - 0.15 (1 - 2 hours). Measure by taking some of the broth out with a sterile DPTP
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