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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃ to -80℃
- 保质期:
12个月
- 英文名:
Recombinant Mouse ICOS Protein (Fc & AVI Tag), Biotinylated
- 库存:
99
- 供应商:
北京义翘神州科技股份有限公司
- 规格:
20 µg/100 µg
| 规格: | 20 µg | 产品价格: | ¥2980.0 |
|---|---|---|---|
| 规格: | 100 µg | 产品价格: | ¥6880.0 |
ICOS重组蛋白|Recombinant Mouse ICOS Protein (Fc & AVI Tag), Biotinylated(产品说明)
蛋白名称:Mouse ICOS Protein (Fc & AVI Tag), Biotinylated
蛋白构建:A DNA sequence encoding the Mouse ICOS (NP_059508.2) (Met1-Leu142) was expressed with a C-terminal Fc region of human IgG1 tag followed by an AVI tag. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.
表达宿主:HEK293 Cells
蛋白纯度:> 90 % as determined by SDS-PAGE.
蛋白活性:Immobilized Recombinant Mouse ICOS Ligand / B7-H2 / ICOSLG Protein (His Tag)(Cat:50190-M08H) at 2 μg/ml (100 μl/well) can bind Recombinant Mouse ICOS Protein (Fc & AVI Tag),Biotinylated(Cat:50466-M41H-B),The EC50 is 60-180 ng/mL.
蛋白内毒素:< 1.0 EU per μg protein as determined by the LAL method.
预测N端:Glu 21
蛋白分子量:The recombinant human ICOS consists of 375 amino acids and predicts a molecular mass of 42.4 kDa.
蛋白NP号:NP_059508.2
蛋白氨基酸序列:Met1-Leu142
蛋白标签:C-human IgG1-Fc & AVI
蛋白名称:Mouse ICOS Protein (Fc & AVI Tag), Biotinylated
蛋白构建:A DNA sequence encoding the Mouse ICOS (NP_059508.2) (Met1-Leu142) was expressed with a C-terminal Fc region of human IgG1 tag followed by an AVI tag. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.
表达宿主:HEK293 Cells
蛋白纯度:> 90 % as determined by SDS-PAGE.
蛋白活性:Immobilized Recombinant Mouse ICOS Ligand / B7-H2 / ICOSLG Protein (His Tag)(Cat:50190-M08H) at 2 μg/ml (100 μl/well) can bind Recombinant Mouse ICOS Protein (Fc & AVI Tag),Biotinylated(Cat:50466-M41H-B),The EC50 is 60-180 ng/mL.
蛋白内毒素:< 1.0 EU per μg protein as determined by the LAL method.
预测N端:Glu 21
蛋白分子量:The recombinant human ICOS consists of 375 amino acids and predicts a molecular mass of 42.4 kDa.
蛋白NP号:NP_059508.2
蛋白氨基酸序列:Met1-Leu142
蛋白标签:C-human IgG1-Fc & AVI
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文献和实验相关实验
研究,其中包括了基因工程单链抗体(single chain Fv,scFv)的应用。一般抗体均大于150KD,不易渗透到组织中,而鼠源抗体用于人体易产生人抗鼠Ig抗体。应用基因工程技术产生的单链抗体是由Ig重链可变区(VH)和轻链可变区(VL)通过一段小肽连接在一起的重组蛋白。分子量仅为27KD,免疫原性降低,易于进入瘤组织,血中半衰期短,易于清除,无Fc段不会与具有Fc受体的正常细胞结合,并且易于产业化。为了提高显像效果Nieroda 用生物反应调节剂 g-干扰素与同位素标记抗体同时使用,促使肿瘤
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Sino Biological 小鼠ICOS重组蛋白 Fc&AVI标签生物素化 共刺激分子icos
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