- 询价
- KA&M BIO
- MAB4927
- 2025年10月12日
- ELISA,IHC,WB
- Mouse
- Aequorea victoria (Water jellyfish) (Mesonema victoria)
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- 详细信息
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- 技术资料
- 亚型：
IgG2a, kappa
- 形态：
Liquid
- 保存条件：
at-20℃
- 适应物种：
Aequorea victoria (Water jellyfish) (Mesonema victoria)
- 库存：
99
- 宿主：
Mouse
- 应用范围：
ELISA,IHC,WB
- 浓度：
见说明
- 抗体英文名：
Anti-GFP Monoclonal antibody (N86/44)
- 规格：
50-100ug
小鼠抗GFP单克隆抗体(克隆号N86/44)上海圻明生物优势供应欢迎咨询。配对抗体小课堂：
Paired antibodies are two antibodies that can bind to an antigenic molecule at the same time. Specifically:
Paired antibodies are commonly used in sandwich ELISA experiments
An antigenic molecule usually has multiple epitopes, and different antibodies can target different epitopes
When two antibodies are able to bind to different epitopes of the same antigenic molecule at the same time, the two antibodies are called paired antibodies
However, not all antibodies against different epitopes can be paired antibodies. Sometimes, when an antibody binds to an antigen, it may cause the configuration of other binding sites to change, making it impossible for other antibodies to bind properly
Steric hindrance effects may also result in two antibodies with close binding sites not being able to bind to the antigen at the same time
Therefore, the preparation of paired antibodies usually requires two steps: first the antibody is prepared, and then the paired antibody is screened
Screening of paired antibodies is typically performed using a sandwich ELISA method of bispecific antibodies, in which different combinations of antibodies are tested to determine which antibodies can be successfully paired
Paired antibodies have important applications in immunology research and diagnostics, especially in assays that require high specificity and sensitivity.
小鼠抗GFP单克隆抗体(克隆号N86/44)更多优势产品欢迎新老客户选购。
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MAB1600 小鼠抗ASFV I10L单克隆抗体(克隆号1A016)
MAB1601 小鼠抗ASFV NS4A单克隆抗体(克隆号1A017)
MAB1602 小鼠抗ASFV NS4A单克隆抗体(克隆号1A018)
MAB1603 小鼠抗ASFV F778R单克隆抗体(克隆号1A019)
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文献和实验[实验原理] 在溶液中的可溶性多价抗原与血清抗体相遇，当两者的比例适当时可以形成一种网状的不溶性的大分子复合物，这叫作免疫沉淀反应。当这样的抗原和相应的抗体在含有电解质的琼脂凝胶中相对扩散时，在抗原与抗体的比例适当处会形成可见的沉淀线，这叫免疫双扩散实验，是免疫沉淀反应的一种。免疫双扩散常用于动物免疫效果的检测和血清抗体效价的测定。本实验使用的粗抗原是大肠杆菌表达的GFP的抽提物，抗体是GFP免疫兔子后得到的抗血清
Immunocytochemistry Protocol Using Anti-GFP Antibodies(使用抗GFP抗体的免疫细胞化学实验方案)
实验试剂 X Dulbecco’s Phosphate-buffered saline (D-PBS) Fixative solution: 4% Formaldehyde solution in PBS, pH 7.4 Permeabilizing solution: 0.25% Triton® X-100 in PBS, pH 7.4 Blocking solution: 5% Normal Goat
小鼠总抗氧化能力的测定 刘小美 宋菊敏 （2006-10-24） 一、原理 机体中有许多抗氧化物质，能使Fe3 ＋ 还原成Fe2 ＋ ，后者可与菲啉类物质形成稳固的络合物，通过比色可测出其抗氧化能力的高低。 二、目的 1．掌握总抗
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