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- 详细信息
- 文献和实验
- 技术资料
- 亚型:
IgG2a, kappa
- 形态:
Liquid
- 保存条件:
at-20℃
- 库存:
99
- 宿主:
Mouse
- 应用范围:
IHC,WB
- 浓度:
见说明
- 抗体英文名:
Anti-FGF13/FHF2 Monoclonal antibody (N225/28)
- 规格:
50-100ug
小鼠抗FGF13/FHF2单克隆抗体(克隆号N225/28)上海圻明生物优势供应欢迎咨询。配对抗体小课堂:
Paired antibodies are two antibodies that can bind to an antigenic molecule at the same time. Specifically:
Paired antibodies are commonly used in sandwich ELISA experiments
An antigenic molecule usually has multiple epitopes, and different antibodies can target different epitopes
When two antibodies are able to bind to different epitopes of the same antigenic molecule at the same time, the two antibodies are called paired antibodies
However, not all antibodies against different epitopes can be paired antibodies. Sometimes, when an antibody binds to an antigen, it may cause the configuration of other binding sites to change, making it impossible for other antibodies to bind properly
Steric hindrance effects may also result in two antibodies with close binding sites not being able to bind to the antigen at the same time
Therefore, the preparation of paired antibodies usually requires two steps: first the antibody is prepared, and then the paired antibody is screened
Screening of paired antibodies is typically performed using a sandwich ELISA method of bispecific antibodies, in which different combinations of antibodies are tested to determine which antibodies can be successfully paired
Paired antibodies have important applications in immunology research and diagnostics, especially in assays that require high specificity and sensitivity.

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文献和实验,Karlsruhe,Germany) 。 ( 3 ) GenePixPro 4.0 软件。 1. 在 FAST 点样基片上的单克隆抗体筛选 ( 1 ) TBS + Tween-20 ( TBST ) :10 mmol/L Tris-HCl,pH 7.5,150 mmol/L NaCl,0.1% ( V/V ) Tween-20。 ( 2 ) 封闭液:2% 牛血清蛋白(BSA ) ( Sigma,St. Louis,MO) 溶于 TBST 中。 ( 3 ) 小鼠抗 RGS
的作用是调控基因表达。例如组蛋白甲基化多导致基因沉默,去甲基化则相反;乙酰化一般是转录激活,去乙酰化则相反。当然,也可在此基础上产生复杂的生物学效应。例如组蛋白去乙酰化酶 HDAC 可影响免疫系统;H3K4me3、H3K9me2 能够调控记忆的形成, 而且 H3K 甲基化与 X 染色体失活、基因组印记和异染色质形成有关;H3 乙酰化通过多种机制调控以来 ATP 的染色质重塑 ,并参与炎症反应;H2A、H2B 泛素化则与 DNA 损害反应有关;而 H3S28 磷酸化与 H3K27 乙酰化可激活转录
5位院士为代表,超12篇CNS大作,5月新冠研究汇总——全方位围攻新冠病毒,值得收藏
的鸡尾酒疗法也引起了大家的关注,不同的抗体可以识别结合域上不同的抗原表位,在未来的临床应用中能够避免免疫逃逸的理想单克隆抗体对。 5 月 8 日,军事医学科学院生物工程研究所陈薇院士团队和西湖大学周强团队强强联合,在预印本平台 bioRxiv 上线题为:A potent neutralizing human antibody reveals the N-terminal domain of the Spike protein of SARS-CoV-2 as a site
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