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- 详细信息
- 文献和实验
- 技术资料
- 亚型:
IgG2a, kappa
- 形态:
Liquid
- 保存条件:
at-20℃
- 库存:
99
- 宿主:
Mouse
- 应用范围:
IHC,WB
- 浓度:
见说明
- 抗体英文名:
Anti-mGluR1/5 (Group I) glutamate receptor Monoclonal antibody (N75/33)
- 规格:
50-100ug
小鼠抗mGluR1/5(克隆号Group I) glutamate receptor单克隆抗体(克隆号N75/33)上海圻明生物优势供应欢迎咨询。配对抗体小课堂:
Paired antibodies are two antibodies that can bind to an antigenic molecule at the same time. Specifically:
Paired antibodies are commonly used in sandwich ELISA experiments
An antigenic molecule usually has multiple epitopes, and different antibodies can target different epitopes
When two antibodies are able to bind to different epitopes of the same antigenic molecule at the same time, the two antibodies are called paired antibodies
However, not all antibodies against different epitopes can be paired antibodies. Sometimes, when an antibody binds to an antigen, it may cause the configuration of other binding sites to change, making it impossible for other antibodies to bind properly
Steric hindrance effects may also result in two antibodies with close binding sites not being able to bind to the antigen at the same time
Therefore, the preparation of paired antibodies usually requires two steps: first the antibody is prepared, and then the paired antibody is screened
Screening of paired antibodies is typically performed using a sandwich ELISA method of bispecific antibodies, in which different combinations of antibodies are tested to determine which antibodies can be successfully paired
Paired antibodies have important applications in immunology research and diagnostics, especially in assays that require high specificity and sensitivity.

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文献和实验viral immunity pathogenesis group
., Schroder, W., Zirngibl, H. and Nieswandt, B. (2000). Structural and functional characterization of the mouse von Willebrand factor receptor GPIb-IX with novel monoclonal antibodies . Blood 95(3): 886-93. Bizzaro, N. and Fiorin, F. (1999
上广泛使用的过继性免疫治疗细胞。 【培养原理】 CIK培养用细胞因子和抗体: n CD3激发型单抗: T细胞活化的第一信号来自于T细胞表面的受体,即T细胞抗原受体(T cell antigen receptor, TCR)与APC提呈的抗原的特异性结合,也就是T细胞对抗原的特异性识别。TCR是由2条不同肽链构成的异二聚体,在T细胞表面,其与CD3分子通过非共价键结合,形成TCR/CD3复合体。TCR识别特异性抗原后会引起CD3和T细胞表面的辅助受体CD4或CD8分子的胞浆尾部聚集
,Karlsruhe,Germany) 。 ( 3 ) GenePixPro 4.0 软件。 1. 在 FAST 点样基片上的单克隆抗体筛选 ( 1 ) TBS + Tween-20 ( TBST ) :10 mmol/L Tris-HCl,pH 7.5,150 mmol/L NaCl,0.1% ( V/V ) Tween-20。 ( 2 ) 封闭液:2% 牛血清蛋白(BSA ) ( Sigma,St. Louis,MO) 溶于 TBST 中。 ( 3 ) 小鼠抗 RGS
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