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- 文献和实验
- 技术资料
- 亚型:
IgG2a, kappa
- 形态:
Liquid
- 保存条件:
at-20℃
- 库存:
99
- 宿主:
Mouse
- 应用范围:
IHC,WB
- 浓度:
见说明
- 抗体英文名:
Anti-BIN1/Amphiphysin-II Monoclonal antibody (N481/113)
- 规格:
50-100ug
小鼠抗BIN1/Amphiphysin-II单克隆抗体(克隆号N481/113)上海圻明生物优势供应欢迎咨询。配对抗体小课堂:
Paired antibodies are two antibodies that can bind to an antigenic molecule at the same time. Specifically:
Paired antibodies are commonly used in sandwich ELISA experiments
An antigenic molecule usually has multiple epitopes, and different antibodies can target different epitopes
When two antibodies are able to bind to different epitopes of the same antigenic molecule at the same time, the two antibodies are called paired antibodies
However, not all antibodies against different epitopes can be paired antibodies. Sometimes, when an antibody binds to an antigen, it may cause the configuration of other binding sites to change, making it impossible for other antibodies to bind properly
Steric hindrance effects may also result in two antibodies with close binding sites not being able to bind to the antigen at the same time
Therefore, the preparation of paired antibodies usually requires two steps: first the antibody is prepared, and then the paired antibody is screened
Screening of paired antibodies is typically performed using a sandwich ELISA method of bispecific antibodies, in which different combinations of antibodies are tested to determine which antibodies can be successfully paired
Paired antibodies have important applications in immunology research and diagnostics, especially in assays that require high specificity and sensitivity.

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文献和实验国自然/高分文章利器!CRISPR文库筛选保姆级指南:从Cas9递送到表型选择一文打通!
研究机构:南开大学 研究人员利用靶向23,439个小鼠基因的全基因组CRISPRa文库,筛选了在LPS刺激下促进B16黑色素瘤细胞转化为MDSC样肿瘤细胞(MLTCs)的关键调控因子。该筛选鉴定出RNA结合蛋白Rbfox3为关键驱动因子。Rbfox3形成相分离的核内液滴,在转录水平抑制Ass1基因的表达,从而发挥免疫抑制功能。天然产物藏红花素可破坏Rbfox3的相分离,抑制MLTC的产生,并增强抗PD-1疗法的疗效。 Adv Sci (Weinh). 2025 Feb;12
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STM:傅阳心、侯玉柱等发现放疗与免疫治疗的交汇点,兵家必争之地竟是这群细胞
细胞或外源性注射 ARTN 同样可以减弱放疗和 PD-L1 阻滞的抗肿瘤作用。上述的结果表明免疫治疗和放疗的治疗作用部分依赖于它们对 Ter 细胞的抑制。 图片来源:Sci. Transl. Med 同样的,破坏 Ter/ARTN 轴能够恢复甚至增加放疗和抗 PD-L1 治疗的疗效。通过使用 anti-Ter119 抗体,将小鼠体内 Ter 细胞耗尽,这显著促进了放疗和抗 PD-L1 治疗的作用。接下来的研究也表明阻断 ARTN 能增强抗肿瘤治疗效果。因此,Ter 细胞耗尽、ARTN 中和都能够
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