- ￥1660 - 10760
- Sino Biological
- 北京
- 11159-H08H
- 2025年08月12日
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- 保存条件：
-20℃ to -80℃
- 保质期：
12个月
- 英文名：
Recombinant Human CTLA4 / CD152 Protein (His Tag)
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 规格：
1.00 mg/100.00 µg/200.00 µg
| 规格： | 1.00 mg | 产品价格： | ￥10760.0 |
|---|---|---|---|
| 规格： | 100.00 µg | 产品价格： | ￥1660.0 |
| 规格： | 200.00 µg | 产品价格： | ￥2570.0 |
CTLA-4重组蛋白|Recombinant Human CTLA4 / CD152 Protein (His Tag)（产品说明）
蛋白名称：Human CTLA4 / CD152 Protein (His Tag)
蛋白构建：A DNA sequence encoding the human CTLA4 (NP005205.2) extracellular domain(Lys36-Phe162) was fused with the a polyhistidine tag at the C-terminus.
表达宿主：HEK293 Cells
蛋白纯度：> 90 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.
蛋白活性：1. Immobilized Recombinant Human CD86 / B7-2 Protein (Fc Tag)(Cat:10699-H02H) at 2 μg/ml (100 μl/well) can bind Recombinant Human CTLA-4 Protein (His Tag) (Cat:11159-H08H), the EC50 is 2.6-8 ng/mL. 2. Measured by its ability to inhibit IL-2 secretion by stimulated Jurkat human acute T cell leukemia cells. The ED50 for this effect is 0.05-0.3μg/mL when stimulated with 1 μg/mL Recombinant Human B7‑1/CD80. 3.Captured Ipilimumab (68052-H001) on proA Chip can bind CTLA-4 (Cat.No.11159-H08H) with an affinity constant of 20.03nM as determined in a SPR assay (Biacore T200)(Routinely tested). 4.Loaded Ipilimumab on ProA Biosensor, can bind CTLA-4 Protein, Human, Recombinant, His Tag(Cat.No.11159-H08H) with an affinity constant of 5.15nM as determined in BLI assay (Sartorius Octet Red384) (Routinely tested). 5.Loaded Recombinant Human B7-1 Protein, hFc Tag (Cat. No. 10698-H02H) on ProA Biosensor, can bind Recombinant Human CTLA-4 Protein, His Tag (Cat. No. 11159-H08H) with an affinity constant of 2.66nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested). 6.Loaded Recombinant Human CD86 Protein, hFc Tag (Cat. No. 10699-H02H) on ProA Biosensor, can bind Recombinant Human CTLA-4 Protein, His Tag (Cat. No. 11159-H08H) with an affinity constant of 5.34nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested).
蛋白内毒素：< 1.0 EU per μg of the protein as determined by the LAL method
预测N端：Lys 36
蛋白分子量：The recombinant human CTLA4 consists of 138 amino acids and has a predicted molecular mass of 15 kDa. As a result of glycosylation, the apparent molecular mass of human CTLA4 is approximately 22-27 kDa in SDS-PAGE under reducing conditions
蛋白NP号：NP_005205.2
蛋白氨基酸序列：Lys36-Phe162
蛋白标签：C-His
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文献和实验1, Waight JD, et al. Selective FcγR Co-engagement on APCs Modulates the Activity of Therapeutic Antibodies Targeting T Cell Antigens.Cancer cell, PubMed ID: 29894690
2, Schofield DJ, et al. Activity of murine surrogate antibodies for durvalumab and tremelimumab lacking effector function and the ability to deplete regulatory T cells in mouse models of cancer.mAbs, PubMed ID: 33397194
》(PNAS)。该论文在线之际，受到国外Scientist，Nature news，The Australian，Thomson Reuters， Fox News， Agence France Presse (AFP法新社) 等美国、英国、俄罗斯、德国、巴西、印度各专业杂志及媒体的广泛关注和报道。 该研究表明由转基因水稻种子生产的重组人血清白蛋白(OsrHSA)在生理生化性质、物理结构，生物学功能、免疫原性与血浆来源的人血清白蛋白一致；并建 立了大规模生产重组人血清白蛋白的生产工艺，获得了高纯度和高
药物研发也已经热潮涌动。2015 年卫计委重大新药创制专项，明确将 PD-1、PD-L1及 CTLA-4 列为重要靶点。申报名单中，中美华世通、中山康方、上海君实、四川大学、复旦大学，目前都还在临床前。名单之外的百济神州，恒瑞以及中信国健也已经在临床前或者临床阶段。 「了解下游层析处理的关键 」 1、什么下游层析处理的关键? 用于肿瘤靶向治疗以及类似以 PD1 类作为靶点的免疫治疗抗体药物作为一种高剂量使用的生物制剂，对产品纯度以及杂质残留量（宿主蛋白 HCP
-1和CTLA-4等“减缓”免疫反应的蛋白质），同时阻断参与免疫系统调节的蛋白质（肿瘤坏死因子，TNF）。国际领先的科学周刊《Nature》在最新一期刊登了这项研究。原文检索：Prophylactic TNF blockade uncouples efficacy and toxicity in dual CTLA-4 and PD-1 immunotherapy③清华大学李丕龙Nature重要发现：独特的转录调控机制生物大分子的“相变”是最近几年生物化学与细胞生物学上一个新兴起的热门研究领域。但人们还不清
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