- ￥3870 - 8940
- Sino Biological
- 北京
- 16014-H49H-B
- 2026年01月26日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃ to -80℃
- 保质期：
12个月
- 英文名：
Recombinant Human LILRB1 Protein (ECD, His & AVI Tag), Biotinylated
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 规格：
20 µg/100 µg
| 规格： | 20 µg | 产品价格： | ￥3870.0 |
|---|---|---|---|
| 规格： | 100 µg | 产品价格： | ￥8940.0 |
ILT2/LILRB1重组蛋白|Recombinant Human LILRB1 Protein (ECD, His & AVI Tag), Biotinylated（产品说明）
蛋白名称：Human LILRB1 Protein (ECD, His & AVI Tag), Biotinylated
蛋白构建：A DNA sequence encoding the Human LILRB1 (ADJ55949.1) (Met1-His458) was expressed with a C-terminal polyhistidine tag followed by an AVI tag. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.
表达宿主：HEK293 Cells
蛋白纯度：≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.
蛋白活性：Immobilized anti-LILRB1, Human IgG4 at 2 μg/mL (100 μL/well) can bind Recombinant Human LILRB1 Protein (ECD, His & AVI Tag), Biotinylated (Cat: 16014-H49H-B), the EC50 is 1.2-3.6 ng/mL.
蛋白内毒素：< 1.0 EU per μg protein as determined by the LAL method.
预测N端：Gly 24
蛋白分子量：The recombinant Human LILRB1 consists of 461 amino acids and predicts a molecular mass of 50.47 kDa. It migrates as an approximately 75.5 kDa band in SDS-PAGE under reducing conditions.
蛋白NP号：ADJ55949.1
蛋白氨基酸序列：Met1-His458
蛋白标签：C-His-AVI
蛋白名称：Human LILRB1 Protein (ECD, His & AVI Tag), Biotinylated
蛋白构建：A DNA sequence encoding the Human LILRB1 (ADJ55949.1) (Met1-His458) was expressed with a C-terminal polyhistidine tag followed by an AVI tag. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.
表达宿主：HEK293 Cells
蛋白纯度：≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.
蛋白活性：Immobilized anti-LILRB1, Human IgG4 at 2 μg/mL (100 μL/well) can bind Recombinant Human LILRB1 Protein (ECD, His & AVI Tag), Biotinylated (Cat: 16014-H49H-B), the EC50 is 1.2-3.6 ng/mL.
蛋白内毒素：< 1.0 EU per μg protein as determined by the LAL method.
预测N端：Gly 24
蛋白分子量：The recombinant Human LILRB1 consists of 461 amino acids and predicts a molecular mass of 50.47 kDa. It migrates as an approximately 75.5 kDa band in SDS-PAGE under reducing conditions.
蛋白NP号：ADJ55949.1
蛋白氨基酸序列：Met1-His458
蛋白标签：C-His-AVI
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文献和实验相关实验
芯片试剂盒里有缓冲液、检测抗体、和带有捕获抗体的膜，这些捕获抗体经过精心选择，有高度特异性，它们在硝酸纤维素膜上重复点样两次。经多项研究证实，蛋白芯片能简化鉴定蛋白所需的筛选过程。通过监控蛋白水平和一些蛋白翻译后修饰的变化，能迅速靶定目标分子，以便进一步的研究。 二、R&D Systems蛋白芯片及其原理试验操作步骤简单介绍 以“检测人的特定位点42种受体酪氨酸激酶（RTKs）的相对磷酸化水平”的Proteome Profiler Phospho-RTK Antibody Array（货号
度，可按图20-4B间接法加生物素化羊抗抗生物素抗血清（biotinylated goat antivaidin antibody）30min室温。倾去加另一层抗生物素-FITC抗血清孵育20min，重复上述1）～3）。 ②AAF标记核酸探针的荧光显示 1）封闭非特异性结合部位：从PBS中取出载片，吸干多余液体，加PBS-0.1%吐温（Tween）含2%正常羊血清（NGS）（5μl/每cm2 盖玻片）。使载片停留在室温5min。 2）倾去多余液体，加抗-AAF抗血清1：750，PBS
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Sino Biological ILT2/LILRB1重组蛋白 生物素化 lilrb1 antibody
￥3870 - 8940
