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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 保质期:
12个月
- 英文名:
Recombinant Lactobacillus casei 60 kDa chaperonin (groL), partial
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
10ug/20ug/50ug/100ug/200ug/500ug/1mg
| 规格: | 10ug | 产品价格: | ¥69.0 |
|---|---|---|---|
| 规格: | 20ug | 产品价格: | ¥139.0 |
| 规格: | 50ug | 产品价格: | ¥209.0 |
| 规格: | 100ug | 产品价格: | ¥349.0 |
| 规格: | 200ug | 产品价格: | ¥419.0 |
| 规格: | 500ug | 产品价格: | ¥559.0 |
| 规格: | 1mg | 产品价格: | ¥699.0 |
Research Areas
Signal TransductionUniprot ID
B3W9W7Gene Names
groELAlternative Name(s)
60 kDa chaperonin;Chaperonin-60;Cpn60Lead Time
3-7 business daysOrganism
Lacticaseibacillus casei (strain BL23) (Lactobacillus casei)Source
YeastExpression Region
182-374aaProtein Length
PartialTag Info
C-terminal 6xHis-taggedTarget Protein Sequence
DTELSVVEGMQFDRGYLSQYMVTDNDKMEADLDDPYILITDKKISNIQDILPLLQEIVQQGKALLIIADDVAGEALPTLVLNKIRGTFNVVAVKAPGFGDRRKAQLEDIATLTGGTVISSDLGLDLKDTKLEQLGRAGKVTVTKDNTTIVDGAGSKDAIAERVNIIKKQIDDTTSDFDREKLQERLAKLAGGVMW
21.9 kDaPurity
Greater than 85% as determined by SDS-PAGE.Form
Lyophilized powder.If the size you ordered does not have lyophilized powder in stock, we will provide it in liquid formBuffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验, Cpn ) Cpn 家族是具有独特的双层 7-9 元环状结构的寡聚蛋白,它们以依赖 ATP 的方式促进体内正常和应急条件下的蛋白质折叠。 Cpns 又分为两组: GroEL(Hsp60) 家族和 TriC 家族。 GroEL 型的 Cpns 存在于真细菌、线粒体和叶绿体中,由双层 7 个亚基组成的圆环组成,每个亚基分子量约为 60Ku 。它们在体
有更好的可溶性。另外,使用NusA系统验证,八个较大分子量蛋白(>70kDa)中的七个得到了可溶性表达,而其它的融合标签(GST,MBP和hexahistidine)系统则只得到了四个可溶性表达的蛋白。 表1. 用大量的目标蛋白评估NusA标签对提高融合蛋白可溶性的作用 参考文献a 目的蛋白数目
作用,或者说它们给处于折叠中间态的多肽链提供了更多的正确折叠的机会,因而它们能提高折叠反应的产率而不一定能提高其速率。细胞内的蛋白质折叠与组装可以发生在胞浆、线粒体或内质网( endoplasmic retinum , ER ),因而分子伴侣可以存在于胞内的各个部位。分子伴侣主要分为以下几类: 1) 伴侣素家族(chaperonin, Cpn) Cpn 家族是具有独特的双层 7-9 元环状结构的寡聚蛋白( Hemminngwen 1988; Cheng 1989 ),它们以依赖 ATP
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