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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Human Probable threonine protease PRSS50 (PRSS50)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Human Probable threonine protease PRSS50 (PRSS50)品名简称:
Recombinant Human PRSS50 protein货号:
CSB-BP883418HU规格:
20μg价格:
3168LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 85% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
PRSS50Uniprot No.:
Q9UI38别名:
Cancer/testis antigen 20;Serine protease 50;Testis-specific protease-like protein 50种属:
Homo sapiens (Human)蛋白长度:
Full Length of Mature Protein来源:
Baculovirus分子量:
41.7 kDa表达区域:
40-385aa氨基酸序列:
AGEAPGALSTADPADQSVQCVPKATCPSSRPRLLWQTPTTQTLPSTTMETQFPVSEGKVDPYRSCGFSYEQDPTLRDPEAVARRWPWMVSVRANGTHICAGTIIASQWVLTVAHCLIWRDVIYSVRVGSPWIDQMTQTASDVPVLQVIMHSRYRAQRFWSWVGQANDIGLLKLKQELKYSNYVRPICLPGTDYVLKDHSRCTVTGWGLSKADGMWPQFRTIQEKEVIILNNKECDNFYHNFTKIPTLVQIIKSQMMCAEDTHREKFCYELTGEPLVCSMEGTWYLVGLVSWGAGCQKSEAPPIYLQVSSYQHWIWDCLNGQALALPAPSRTLLLALPLPLSLLAAL蛋白标签:
C-terminal 6xHis-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
28-38 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
May be involved in proteolysis through its threonine endopeptidase activity.功能2:
/文献:
/研究领域:
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文献和实验Chromosome Conformation Capture (3C) (PROT05)
final); Spin down for 15 minutes at 3500rpm. Lysis The cells are lysed to isolate nuclei. Resuspend cells in 50ml of lysis buffer with freshly added protease inhibitors. Add a small magnetic stirrer and incubate on ice for 90
and centrifuge 4 minutes at 2000 RPM;Remove the PBS+1/1000 PMSF by aspiration and re-suspend cell pellet in 1ml ChIP lysis buffer plus the protease inhibitors 1/1000 PMSF and 50 units of RNAse inhibitor. The final volume of cell lysis buffer should be sufficient
TSA Treatment of Mammalian Cells (PROT09)
A) from SIGMA (T8552). Stock solution at 1mg/ml in ethanol (storage at -20°) . The cells (20% of confluence) are allowed to grown for 24 hours attached on the plate. Add the drug in complete medium. Various doses are tested to define optimal conditions
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