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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Human Tissue factor (F3), partial (Active)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Human Tissue factor (F3), partial (Active)品名简称:
Recombinant Human F3 protein, partial (Active)货号:
CSB-MP007928HU2规格:
20μg价格:
828LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 95% as determined by SDS-PAGE.内毒素:
Less than 1.0 EU/μg as determined by LAL method.生物活性:
Measured by its binding ability in a functional ELISA. Immobilized Human TF at 2 μg/mL can bind Anti-TF recombinant antibody (CSB-RA007928MA2HU). The EC50 is 1.434-1.635 ng/mL.基因名:
F3Uniprot No.:
P13726别名:
/种属:
Homo sapiens (Human)蛋白长度:
Partial来源:
Mammalian cell分子量:
26.2 kDa表达区域:
33-251aa氨基酸序列:
SGTTNTVAAYNLTWKSTNFKTILEWEPKPVNQVYTVQISTKSGDWKSKCFYTTDTECDLTDEIVKDVKQTYLARVFSYPAGNVESTGSAGEPLYENSPEFTPYLETNLGQPTIQSFEQVGTKVNVTVEDERTLVRRNNTFLSLRDVFGKDLIYTLYYWKSSSSGKKTAKTNTNEFLIDVDKGENYCFSVQAVIPSRTVNRKSTDSPVECMGQEKGEFRE蛋白标签:
C-terminal 10xHis-tagged产品提供形式:
Lyophilized powder缓冲液:
Lyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
3-7 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
Initiates blood coagulation by forming a complex with circulating factor VII or VIIa. The [TF:VIIa] complex activates factors IX or X by specific limited proteolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade.功能2:
/文献:
Human tissue factor: cDNA sequence and chromosome localization of the gene. Scarpati E.M., Wen D., Broze G.J. Jr., Miletich J.P., Flandermeyer R.R., Siegel N.R., Sadler J.E. Biochemistry 26:5234-5238 (1987)研究领域:
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文献和实验Genes Involved in the Metastatic Cascade of Medullary Thyroid Tumours
is partly due to the lack of appropriate animal models from which to investigate this complex process. The retinoblastoma (Rb) tumour suppressor pathway (pRb/E2F) is mutated in almost all human tumours, and a number of laboratories have now established pRb- or E2F-deficient
胰腺上皮内瘤变和胰腺癌组织中TGF-f3/Smad信号通路相关蛋白表达
。病理上属于高分化的有21例,中分化68例,低分化32例。 1.2 组织芯片构建 找出存在PanlN病灶的HE切片和相应存档的组织蜡块,先在切片PanlN病灶处用记号笔作好标记,比对蜡块,在对应点同样作好标记。组织芯片构建参照Kononen等建立的方法,应用组织芯片构建仪(Tissue Arrayer,Beecher Instruments,Silver Spring,MD,USA)制备组织芯片。流程如下:应用构建仪在预先制备的空白蜡块(45 mm x20 mm)中穿孑L(直径2 mm,深度
Phosphopeptide mapping of proteins ectopically expressed in tissue culture cell lines
, we employed metabolic cell labeling with 32P of tissue culture cells coexpressing a Flag-epitope tagged HAND1 along with a number of active kinases and phosphatase subunits. We generated phosphopeptide maps of the phosphorylated HAND1 using the methods
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