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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Human Mucin-13(MUC13), partial (Active)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
1mg/100μg/20μg
| 规格: | 1mg | 产品价格: | ¥27960.0 |
|---|---|---|---|
| 规格: | 100μg | 产品价格: | ¥6654.0 |
| 规格: | 20μg | 产品价格: | ¥2376.0 |
纯度:
Greater than 95% as determined by SDS-PAGE.基因名:
MUC13Uniprot No.:
Q9H3R2别名:
DRCC1, RECC种属:
Homo sapiens (Human)蛋白长度:
Partial来源:
Mammalian cell分子量:
44.0 kDa表达区域:
19-421aa氨基酸序列:
TNQGNSADAVTTTETATSGPTVAAADTTETNFPETASTTANTPSFPTATSPAPPIISTHSSSTIPTPAPPIISTHSSSTIPIPTAADSESTTNVNSLATSDIITASSPNDGLITMVPSETQSNNEMSPTTEDNQSSGPPTGTALLETSTLNSTGPSNPCQDDPCADNSLCVKLHNTSFCLCLEGYYYNSSTCKKGKVFPGKISVTVSETFDPEEKHSMAYQDLHSEITSLFKDVFGTSVYGQTVILTVSTSLSPRSEMRADDKFVNVTIVTILAETTSDNEKTVTEKINKAIRSSSSNFLNYDLTLRCDYYGCNQTADDCLNGLACDCKSDLQRPNPQSPFCVASSLKCPDACNAQHKQCLIKKSGGAPECACVPGYQEDANGNCQKCAFGYSGLDCKDKFQL蛋白标签:
C-terminal 10xHis-tagged产品提供形式:
Lyophilized powder缓冲液:
Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4.货期:
3-7 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能:
Epithelial and hemopoietic transmembrane mucin that may play a role in cell signaling.内毒素:
Less than 1.0 EU/ug as determined by LAL method.SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.LC-MS Image Description/Western Blot:
/Product types:
Active Protein & In Stock ProteinBiological_Activity:
Measured by its binding ability in a functional ELISA. Immobilized Human MUC13 at 2 μg/mL can bind Anti-MUC13 recombinant antibody (CSB-RA887973MA1HU). The EC50 is 3.806-5.269 ng/mL.Research Areas:
Signal TransductionReconstitution:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.Reference:
solation and characterization of a novel human gene DRCC1 encoding a mucin-like glycoprotein, homologous to murine cell surface antigen 114/A10, and its reduced expression in colorectal cancers. Masayoshi I., Furukawa Y., Akashi H., Han H., Nakajima Y., Sugano S., Ogawa M., Nakamura Y. Submitted to EMBL/GenBank/DDBJ databases (DEC-1999)Function:
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文献和实验Active Motif ChIP 技术专家经验分享:如何才能做好 ChIP 实验?
量的 ChIP 数据却非常具有挑战性。在每个关键步骤都有很多需要注意的细节。下面是 Active Motif ChIP 实验专家根据多年经验总结的每个步骤中的原理及一些需要注意的细节。第一步 染色质交联为什么要用甲醛交联?ChIP 技术主要是一种解码信息(目的蛋白在染色质上的分布)的方法。对于解码信息,首先就需要确保信息保存下来而不失真,ChIP 技术用到的最主要的信息保存方式是甲醛交联。甲醛交联也是 ChIP 实验非常关键的一个步骤。最早的 ChIP 技术是不用甲醛交联的[6],只是后来发现甲醛交联能更好的保存蛋白
Cultures of Proliferating Vascular Smooth Muscle Cells from Adult Human Aorta
Abnormal proliferation of human vascular smooth muscle cells (hVSMCs) is a central event in the development of atherosclerosis (1 –3 ) As a result, there is considerable interest in the establishment of hVSMC cultures as a mode
in the red blood cells from different species (13 –15 ). There are a total of six cysteine residues in the human hemoglobin molecule, but two of them (β93 residues) are highly reactive (16 ) and can form mixed disulfide with GSH (14
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