dam蛋白|大肠杆菌dam蛋白|大肠杆菌dam重组蛋白|Es

Research Areas

Others

Uniprot ID

P0AEE8

Gene Names

dam

Alternative Name(s)

(DNA adenine methyltransferase)(Deoxyadenosyl-methyltransferase)(Orphan methyltransferase M.EcoKDam)(M.EcoKDam)

Lead Time

3-7 business days

Organism

Escherichia coli (strain K12)

Source

E.coli

Expression Region

1-278aa

Protein Length

Full Length

Tag Info

N-terminal 6xHis-tagged and C-terminal Avi-tagged

Target Protein Sequence

MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLNTDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQDPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFFYCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVERHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPAKK

MW

41.4 kDa

Purity

Greater than 85% as determined by SDS-PAGE.

Endotoxin

/

Biological_Activity

/

Form

Liquid or Lyophilized powder

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Storage

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.

Relevance

An alpha subtype methylase that recognizes the double-stranded sequence 5'-GATC-3' and methylates A-2 on both strands (Probable). Although it shares sequence specificity with a number of type II restriction endonucleases and methylases, it is thought to act in methyl-directed mismatch repair, initiation of chromosome replication and gene expression.

Function

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