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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃/-80℃
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Dermatophagoides pteronyssinus Mite allergen Der p 6 (DERP6) (X4S), partial
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
1mg/100ug/20ug
| 规格: | 1mg | 产品价格: | ¥17754.0 |
|---|---|---|---|
| 规格: | 100ug | 产品价格: | ¥4164.0 |
| 规格: | 20ug | 产品价格: | ¥2208.0 |
Research Areas
OthersUniprot ID
P49277Gene Names
DERP6Alternative Name(s)
Allergen Der p VI DP5 Allergen: Der p 6Lead Time
3-7 business daysOrganism
Dermatophagoides pteronyssinus (European house dust mite)Source
YeastExpression Region
1-20aa(X4S)Protein Length
Full LengthTag Info
C-terminal hFc-taggedTarget Protein Sequence
AIGSQPAAEAEAPFQISLMKMW
28.7 kDaPurity
Greater than 90% as determined by SDS-PAGE.Endotoxin
/Biological_Activity
/Form
Liquid or Lyophilized powderBuffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.Storage
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.Relevance
Protease that shows specificity similar to chymotrypsin.Function
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文献和实验为非溶性蛋白,也可被单克隆抗体柱层析,重新还原的产物可以被抗Derpl血清识别,其结合率优于pGEX克隆产物。为了减少繁琐,表达更好的重组蛋白,有人尝试以Der p1的原酶和酶原放人同一酵母载体表达,效果良好。 3.哺乳动物表达 构建一个扩大的载体PEE6.HCMV.GS,从中国仓鼠卵巢细胞(CHO)表达Derpl和Derp2。人巨细胞病毒(CMV)启动子(ACMD)和谷胱甘肽合成酶基因,通过放大质粒拷贝数增加细胞内酶高效表达,以控制抗代谢产物硫代
,因为该交联物能诱导B细胞只产生IgG而非IgE,结果导向无病理变化的免疫效应过程,该疫苗对于哮喘的预防十分有益。这类佐剂包括几-12等。实验证明,经卵白蛋白交联IL-12免疫小鼠后,可以抑制IgE的产生;质粒或裸DNA疫苗也可诱导Thl细胞反应。令人惊奇的是p半乳糖苷酶结合人CMV载体免疫小鼠也可诱导产生IgG2。,而非IgE,甚至可抑制IgE的再次反应,故该系统克隆的重组蛋白(Der p5)可降低支气管组胺的水平,抑制IgE效应以及气道高反应性。裸DNA疫苗通过呈递HLA-I类分子以及诱导产生IL
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