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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 保质期:
12个月
- 英文名:
Recombinant Bovine leukemia virus Envelope glycoprotein (env), partial
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
10ug/20ug/50ug/100ug/200ug/500ug/1mg
| 规格: | 10ug | 产品价格: | ¥69.0 |
|---|---|---|---|
| 规格: | 20ug | 产品价格: | ¥139.0 |
| 规格: | 50ug | 产品价格: | ¥209.0 |
| 规格: | 100ug | 产品价格: | ¥349.0 |
| 规格: | 200ug | 产品价格: | ¥419.0 |
| 规格: | 500ug | 产品价格: | ¥559.0 |
| 规格: | 1mg | 产品价格: | ¥699.0 |
Research Areas
OthersUniprot ID
P25057Gene Names
envAlternative Name(s)
Env polyprotein;SU;Glycoprotein 51;gp51;TM;Glycoprotein 30;gp30Lead Time
3-7 business daysOrganism
Bovine leukemia virus (isolate Australian) (BLV)Source
Mammalian cellExpression Region
34-301aaProtein Length
PartialTag Info
C-terminal hFc-taggedTarget Protein Sequence
WRCSLSLGNQQWMTAYNQEAKFSISIDQILEAHNQSPFCAKSPRYTLDSVNGYPKIYWPPPQGRRRFGARAMVTYDCEPRCPYVGADRFDCPHWDNASQADQGSFYVNHQILFLHLKQCHGIFTLTWEIWGYDPLITFSLHKIPDPPQPDFPQLNSDWVPSVRSWALLLNQTARAFPDCAICWEPSPPWAPEILVYNKTISSSGPGLALPDAQIFWVNTSSFNTTQGWHHPSQRLLFNVSQGNALLLPPISLVNLSTASSAPPTRVRRMW
59.3 kDaPurity
Greater than 95% as determined by SDS-PAGE.Form
Lyophilized powder.If the size you ordered does not have lyophilized powder in stock, we will provide it in liquid formBuffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验以上阴性对照的OD值>0.1,应重复实验。)。样品OD值<临界值者为HIV抗体阴性。 (4)、正常情况下,阳性对照孔的OD值≥0.80(若1孔阳性对照的OD值<0.8应舍弃,若有2孔或2孔以上阳性对照的OD值<0.8,应重复实验。样品OD值≥临界值者为HIV抗体阳性。 3、HIV测定(蛋白印迹法)结果的判定方法 根据卫生部1990(2)号文件的规定,蛋白印迹法的判断标准为: (1)、阳性:至少有一条env(gp160,gp120,gp41)带和一条pol(p65,p51,p32)带
LINEAGE ANALYSIS USING RETROVIRAL VECTORS
to naturally occurring retrovirus particles. They enter the host cell via interaction of a viral envelope glycoprotein (a product of the viral env gene) with a host cell receptor. The murine viruses have several classes of env glycoprotein which interact
,将血凝素成功地嵌入病毒外壳,并能有效感染人和鸟类细胞。Jane等用泡状口炎病毒G糖蛋白取代莫洛尼病毒env蛋白,使该病毒颗粒能成功地感染非哺乳动物如Zebra fish细胞,更重要的是这种病毒外壳可耐受超离心而不影响感染效率,因此有利于浓缩病毒,提高滴度。 (二)目的基因组织专一性表达的调节 许多组织专一性表达的调节片段已研究清楚,把这些片段与目的基因一起重组入逆转录病毒可用于基因的靶向表达。 1、肝专一性表达 肝专一性表达研究较多的是磷酸烯醇式酮酸
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