CTLA4基因敲除质粒

pLenti-CTLA4-sgRNA (CTLA4基因敲除质粒)是一种在动物细胞中可以同时表达Cas9、目的基因的sgRNA和puromycin抗性基因的质粒。用于在动物细胞中直接基于CRISPR/Cas9技术敲除目的基因，或者通过包装慢病毒后基于CRISPR/Cas9技术敲除目的基因。本质粒中sgRNA的有效性已经通过T7EI法的验证。

本质粒在细菌中为Amp抗性，全长约13,000bp。本质粒的关键图谱信息请参考图1。本质粒可直接转染细胞用于目的基因的CRISPR/Cas9敲除，以及通过puromycin筛选稳定细胞株；也可以与pMDLg、Rev及VSV-g共转HEK293T细胞进行重组慢病毒(lentivirus)的包装，然后再用于感染细胞或组织并进行目的基因的CRISPR/Cas9敲除。

图1. 表达sgRNA、Cas9和puromycin抗性的pLenti-sgRNA质粒关键图谱信息。

本质粒中的sgRNA基于碧云天研发的CRISPR/Cas9 sgRNA快速筛选和验证体系获得，sgRNA的有效性已经通过T7EI法验证。

本质粒用于实验时，建议同时选购无任何靶向的对照质粒pLenti-Control-sgRNA (L00011)或靶向GFP的对照质粒pLenti-GFP-sgRNA (L00013)。

碧云天同时提供基于CRISPR/Cas9技术的CTLA4基因敲除的质粒(L25770 pLenti-CTLA4-sgRNA)、慢病毒(L25771 CTLA4 Knockout Lentivirus)、HEK293T细胞(L25772 CTLA4 Knockout HEK293T Cells)、HEK293T敲除细胞的RIPA裂解液(L25773 CTLA4 Knockout HEK293T RIPA Lysate)、HEK293T敲除细胞的Trizol裂解液(L25774 CTLA4 Knockout HEK293T Trizol Lysate)等产品，具体请在碧云天网站查询或在本产品网页点击相应产品。

CTLA4基因的基本信息如下：

Species	Gene Symbol	Gene ID	GenBank Accession	Transcript
Human	CTLA4	1493	BC069566	NM_005214

 

About the gene
Official Symbol	CTLA4
Previous Symbol	CELIAC3; IDDM12
Official Full Name	cytotoxic T-lymphocyte associated protein 4
Synonyms	CD152; CD; GSE; CTLA-4
Location	2q33.2
Gene Type	protein_coding
Uniprot ID	P16410.3
Pathway/Library	others
Gene Summary	This gene is a member of the immunoglobulin superfamily and encodes a protein which transmits an inhibitory signal to T cells. The protein contains a V domain, a transmembrane domain, and a cytoplasmic tail. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. The membrane-bound isoform functions as a homodimer interconnected by a disulfide bond, while the soluble isoform functions as a monomer. Mutations in this gene have been associated with insulin-dependent diabetes mellitus, Graves disease, Hashimoto thyroiditis, celiac disease, systemic lupus erythematosus, thyroid-associated orbitopathy, and other autoimmune diseases.