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Recombinant Human REV1 Polymer

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  • ￥1080 - 45885
  • Prospecbio
  • 以色列
  • enz-796
  • 2026年01月08日
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    上海经科化学科技有限公司
    16 年钻石会员

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    • 文献和实验
    • 技术资料
    • 保存条件：

      for future use below -18°C

    • 保质期：

      See instructions

    • 英文名：

      REV1

    • 库存：

      常规产品有备货

    • 供应商：

      上海经科化学科技有限公司

    • CAS号：

      无

    • 规格：

      5ug/20ug/1mg

    规格：5ug产品价格：￥1080.0
    规格：20ug产品价格：￥2415.0
    规格：1mg产品价格：￥45885.0

    产品细节图片1

    CATALOGUE NUMBER

    ENZ-796

    SYNONYMS

    REV1 Polymerase (DNA Directed), REV1L, Alpha Integrin-Binding Protein 80, Rev1-Like Terminal Deoxycytidyl Transferase, AIBP80, REV1 (Yeast Homolog)- Like, REV1 Homolog (S. Cerevisiae) , REV1-Like (Yeast) , DNA Repair Protein REV1, REV1 Homolog, REV1- Like, EC 2.7.7.-.

    INTRODUCTION

    REV1 Polymerase (REV1) contains a BRCT domain, which is imperative in protein-protein interactions. REV1 is a deoxycytidyl transferase involved in DNA repair. REV1 assists in the 1st step in the bypass of abasic lesions by the incorporation of a nucleotide opposite the lesion. REV1 is essential for normal generation of mutations by physical and chemical agents. REV1 transfers a dCMP residue from dCTP to the 3'-end of a DNA primer in a template-dependent reaction. A role for the human Rev1-like protein has been suggested as a scaffold which recruits DNA polymerases involved in translesion synthesis (TLS) of damaged DNA.

    DESCRIPTION

    REV1 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 227 amino acids (51-256) and having a molecular mass of 25.2kDa.
    REV1 is fused to a 21 amino acid His-tag at N-terminus.

    SOURCE

    Escherichia Coli.

    PHYSICAL APPEARANCE

    Sterile Filtered colorless solution.

    FORMULATION

    The REV1 solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.4M Urea and 10% glycerol.

    STABILITY

    Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
    For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
    Avoid multiple freeze-thaw cycles.

    PURITY

    Greater than 85.0% as determined by SDS-PAGE.

    AMINO ACID SEQUENCE

    MG./S-SHHHHHH SSGLVPRGSH MGVAIYVNGY TDPSAEELRK LMMLHGGQYH VYYSRSKTTH IIATNLPNAK IKELKGEKVI RPEWIVESIK AGRLLSYIPY QLYTKQSSVQ KGLSFNPVCR PEDPLPGPSN IAKQLNNRVN HIVKKIETEN EVKVNGMNSW NEEDENNDFS FVDLEQTSPG RKQNGIPHPR GSTAIFNGHT PSSNGALKTQ DCLVPMVNSV ASRLSPA.

    SAFETY DATA SHEET

    SDS

    USAGE

    ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
     

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    图标文献和实验
    该产品被引用文献

    REV1 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 227 amino acids (51-256) and having a molecular mass of 25.2kDa.
    REV1 is fused to a 21 amino acid His-tag at N-terminus.

    相关实验

    • Screening Recombinant Libraries by Polymerase Chain Reaction

      The complexity of the genome of a particular organism or the relative abundance of a particular mRNA, within the cell type from which a cDNA library was constructed, affects the ability with which one can isolate a gene or cDNA clone

    • Construction of Recombinant Human Cytomegalovirus

      The use of reverse genetics to generate recombinant viruses allows the researcher to investigate the exact functional significance of particular viral genes during the virus life cycle, by means of their deletion or modification in the viral

    • Use of Polymerase Chain Reaction for Making Recombinant Constructs

      The capacity to recombine and modify DNA are underpinnings of the recombinant DNA revolution. The polymerase chain reaction (PCR) (1 ,2 ) provides a rapid means for the site-directed mutagenesis of DNA and for the recombination of DNA

    查看更多相关实验 >

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    文献支持
    Recombinant Human REV1 Polymerase
    ￥1080 - 45885