- ￥900
- AAB
- 上海
- A21540
- 2025年07月14日
- WB,IHC-p,ELISA
- Rabbit
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Synthesized peptide derived from Sialic acid-binding Ig-like lectin 5/Sialic acid-binding Ig-like lectin 14 at AA range: 91-140
- 形态：
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 保存条件：
-20°C/1 year
- 克隆性：
Polyclonal
- 适应物种：
Human
- 保质期：
1年
- 宿主：
Rabbit
- 应用范围：
WB,IHC-p,ELISA
- 浓度：
1 mg/ml
- 靶点：
SIGLEC5/SIGLEC14
- 抗体英文名：
Siglec-5/14 rabbit pAb
- 抗体名：
Sialic acid-binding Ig-like lectin 5/Sialic acid-binding Ig-like lectin 14
- 规格：
50ul
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文献和实验ELISA (Enzyme-Linked ImmunoadSorbent Assay)
and PSAR (Peroxidase-conjugated Swine Anti Rabbit) for Ab raised in rabbits. 10) Wash 4x with 1x PBS containing 0.01% tween. 11) Add substrate (100ul/well). 12) Incubate for 1-5 mins in the dark at RT. Check reaction for colour change. Wells
(交流)ELISA (Enzyme-Linked ImmunoadSorbent Assay)
in rabbits.10) Wash 4x with 1x PBS containing 0.01% tween.11) Add substrate (100ul/well).12) Incubate for 1-5 mins in the dark at RT. Check reaction for colour change. Wells should turn blue.13) Stop reaction by the addition of 25ul 0.1M H2SO4/well.14) Read
Western blotting using ECL reagent
membrane (Amersham Life Science; cat # RPN2020D)Kodak X-OMAT (XAR-5, 18X24 cm; cat # 8532665)10X TBS12.11 g Tris-base (100 mM)87.66 g NaCl (1500 mM)1 liter dd H2OAdjust pH= 7.6Washing buffer (TBS-T): 100 ml 10X TBS + 900 ml dd H2O + 1 ml Tween-20.Blocking
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