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- 文献和实验
- 技术资料
- 免疫原:
The antiserum was produced against synthesized peptide derived from human p70 S6 Kinase beta around the phosphorylation site of Ser423. AA range:389-438
- 形态:
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 保存条件:
-20°C/1 year
- 克隆性:
Polyclonal
- 适应物种:
Human,Mouse
- 保质期:
1年
- 宿主:
Rabbit
- 应用范围:
WB,ELISA
- 浓度:
1 mg/ml
- 靶点:
RPS6KB2
- 抗体英文名:
p70 S6 kinase β (phospho Ser423) rabbit pAb
- 抗体名:
Ribosomal protein S6 kinase beta-2
- 规格:
50ul
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文献和实验at 10, 25, and 50 μM). At 24 h post-treatment, celllysates were obtained; SDS-PAGE and Western blotting were performed as described in Section 3, Methods. AR-A014418dose-dependent decreases of phospho-β-catenin (Ser33/37) and phospho-glycogen synthase
v468. chapter 4 WNT 细胞通路 检测细胞GSK3活性与表达水平
a pseudo-substrate and binds to the phosphate-binding pocketon GSK3, preventing interaction with primed substrates (8) . Phosphorylation at this site is mediated by members of the AGCfamily of kinases (e.g. PKB, PKC, p70S6K, and p90RSK) andcommonly
提示我模型中的通路究竟涉及哪个亚型,而我又没有那么多经费一个一个亚型去试,故我想能不能就检测总的PKC激活水平,毕竟有亚型被激活,那总的活化PKC水平也应该会升高啊!虽然敏感性和特异性肯定没有单独检测一个亚型的高。 还想请教:Phospho-PKC (pan) (gamma Thr514) Antibody 和Phospho-PKC (pan) (βII Ser660) Antibody 这两个抗体的针对的亚型有什么不同?既然都是pan,那是否说都是针对所有的亚型,只是磷酸化的残基不同
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