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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
The antiserum was produced against synthesized peptide derived from human CHST10. AA range:191-240
- 形态:
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 保存条件:
-20°C/1 year
- 克隆性:
Polyclonal
- 适应物种:
Human,Mouse,Rat
- 保质期:
1年
- 宿主:
Rabbit
- 应用范围:
WB,IHC-p,ELISA
- 浓度:
1 mg/ml
- 靶点:
CHST10
- 抗体英文名:
HNK-1ST rabbit pAb
- 抗体名:
Carbohydrate sulfotransferase 10
- 规格:
50ul
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文献和实验= NAL), appears to form upon oxidative cyclization of the nonfluorescent 2:1 lysine-HNE Michael adduct-Schiff base cross-link (Scheme 1). Polyclonal antibody (PAb) to the NAL-HNE fluorophore was raised in rabbit and found to be highly specific
GST-fusion protein injection into rabbits
injection can be done. Blood is collected and sera is analyzed see below. 7. Collect the entire blood from the rabbit (~150-200 ml = 4th and final bleed). Analyze each pre-immune sera and various bleeds from 1st, 2 nd, 3rd, 4th at 1:500
RUVKUN Antibody Staining Protocol revised 10/29/90
hours at RT. 13. Wash for 4-24 hours at RT in Buffer B (usually do 5 quick washes and then 2-3 hours later do 1-2 more). 14. Incubate with 2 in Buffer A, 4-24 hours, RT (1:200 FITC-coupled goat anti-rabbit antibody for lin-14
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