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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Synthesized peptide derived from human protein . at AA range: 720-800
- 形态:
Liquid in PBS containing 50% glycerol, and 0.02% sodium azide.
- 保存条件:
-20°C/1 year
- 克隆性:
Polyclonal
- 适应物种:
Human
- 保质期:
1年
- 宿主:
Rabbit
- 应用范围:
WB,ELISA
- 浓度:
1 mg/ml
- 靶点:
GPR128
- 抗体英文名:
GP128 rabbit pAb
- 抗体名:
Probable G-protein coupled receptor 128
- 规格:
50ul
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文献和实验= NAL), appears to form upon oxidative cyclization of the nonfluorescent 2:1 lysine-HNE Michael adduct-Schiff base cross-link (Scheme 1). Polyclonal antibody (PAb) to the NAL-HNE fluorophore was raised in rabbit and found to be highly specific
Immunoflourescence of Cultured Cells
Materials L-lysine coated glass cover slips or charged glass slides Neutral Buffered Formalin (Sigma HT50-128) 0.5% NP40 in PBS (2.5 N HCl or 0.07 N NaOH for BrDU staining only) Primary and secondary
Making antibodies to synthetic pepti
at once. We shave the upper back of the rabbit prior to immunization. We tend to use 1 mg of peptide per immunization (3-5 intradermal sites at each immunization), and re-immunize at 2-6 week intervals. Intradermal (intracutaneous) means: raise a wheal (inject
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