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MINICHROMOSOME MICROTUBULE BINDING ASSAY
the inhibitor in the YWB. Let sit at room temperature for 2-3 min. Add oxalyticase [12uL 1mg/ml stock]. Incubate at 23o C with gentle shaking to form spheroplasts. Spheroplasting should be complete within one hour; avoid incubations longer than 1h, 15min
Characterization of the Effects of RanGTP on the Microtubule Cytoskeleton
assembly and maintenance in mitosis (2-10). In addition, Ran regulates nuclear-membrane assembly upon exit from mitosis (11,12). This chapter focuses on the regulatory role of Ran in spindle assembly and maintenance, outlining techniques used to study
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