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- 2025年12月05日
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文献和实验1、引物设计由美国Perkin-elmer公司合成，引物末端标记有生物素（1）扩增体系10×PCR buffer 5μlPCR引物 10μl4×dNTP 10μlTaq polymerase 0.25μlDNA模板 10μlD、W 14.75μ
Knowledge-Based Virtual Screening: Application to the MDM4/p53 ProteinProtein Interaction
or conserved molecular recognition. Herein, we demonstrate the potential of knowledge-based virtual screening by applying it to the MDM4-p53 protein–protein interaction where the MDM2-p53 protein–protein interaction constitutes the parent reference
target, and the process of structure-based drug design. A review of purine recognition in representatives of the various purine-binding protein families, as well as the challenges faced in targeting members of the purinome in drug discovery campaigns
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