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文献和实验Acid Phosphatase for Glycol Methacrylate Sections Procedure
, sodium salt (Sigma) - 40 mg N.N-dimethylformamide - 4 ml Pararosaniline Solution Pararosaniline (C.I.# 42500) - 2 g 2N HCl - 50 ml Use heat to dissolve, filter when cool and store at 4°C. Sodium Nitrite Solution
Acid Phosphatase for Glycol Methacrylate Sections Procedure
Naphtol AS-BI phosphatease, sodium salt (Sigma) - 40 mg N.N-dimethylformamide - 4 ml Pararosaniline Solution Pararosaniline (C.I.# 42500) - 2 g 2N HCl - 50 ml Use heat to dissolve, filter when cool and store at 4°C
Quantitation of Protein:Bicinchoninic Acid (BCA) Method
of amino acids can bind with Cu 2+ to form a complex in the basic solution and then the Cu 2+ is deoxidized to Cu + . Bicinchoninic acid and its sodium salt are water-soluble. In the alkaline environment, it can bind with Cu
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