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文献和实验A Homogeneous Assay to Assess GABA Transporter Activity
a convenient functional uptake assay for GABA transport into cell lines transiently transfected with GABA transporter?1 (GAT?1) and other GAT isoforms. This facile, homogeneous assay allows for the determination of K m , V max , and K i values. The assay
. The ultimate goal of siRNA experiments is to assign gene function, analyze biological pathways, or validate potential drug targets. But before that goal can be realized, the siRNA has to be designed, synthesized, delivered to cells using an optimized protocol
?based high?throughput fluorescence resonance energy transfer (FRET) HCV infection screening assay, which allows for the identification of inhibitors that target HCV at any step in the viral life cycle. Basic protocols are provided for compound screening
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