Lipid Droplets Fluorescence As

Cayman的脂滴荧光检测试剂盒可用于研究脂滴生物合成的调节剂。该检测的主要优点是尼罗红的绿色荧光对脂滴非常敏感且具有特异性。此外，各种细胞类型对不同操作的脂滴生物合成变化可以通过荧光显微镜、流式细胞术或荧光板读数器进行定性和定量。当与与兼容荧光团偶联的抗体一起使用时，还可以同时可视化脂滴和相关蛋白。油酸通常用于诱导培养细胞中的脂滴形成，作为阳性对照。当以96孔板形式使用时，该试剂盒提供足够的试剂来有效处理/染色480个单个细胞孔。Cayman's Lipid Droplets Fluorescence Assay Kit can be used to study regulators of lipid droplet biogenesis. The main advantage of this assay is that the green fluorescence of Nile Red is both very sensitive and specific for lipid droplets. Furthermore, changes in lipid droplet biogenesis in various cell types in response to different manipulations can be both qualified and quantified by fluorescence microscopy, flow cytometry, or fluorescent plate readers. Simultaneous visualization of lipid droplets and associated proteins is also possible when used with antibodies conjugated to compatible fluorophores. Oleic acid, which is commonly used to induce lipid droplet formation in cultured cells, is included as a positive control. This kit provides sufficient reagents to effectively treat/stain 480 individual wells of cells when utilized in a 96-well plate format.分子式分子量