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- 2025年07月03日
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文献和实验Detection of apoptotic process in situ using immunocytochemical and TUNEL assays
The immunocytochemical procedure described represents an original modification of the method for cell death detection by ELISA. Utilizing this new modified assay we are able to show the presence of apoptotic cells or cells involved in apoptosis in the thymus
Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
to establish, by using separate assays, that the interacting regions captured in the 6C assay are truly occupied by the protein of interest in cells. To this end, perform ChIP assays (Tiwari et al. 2008) using antibodies specific for the protein
in the nucleus in human cell cultures we designed several RNA-biotin based pulldown assays which can be used alone or in combination with other known assays such as ChIP and Flag-tagged pulldown assays. Three protocols are explained
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