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999
- 规格:
100 ul
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文献和实验Steady State ATPase Assays Coupled Enzyme System
Motor protein (>95% purity; 15-20 µM) Cuvettes (200 µL volume, 10 mm path length) 0.5 M Tris-OAc, pH 7.5 10 mM MgCl2 10 mM DTT
ADENOSINE TRIPHOSPHATASE (ATPASE)
and 4.6 Preincubate at 4C in 0.1M sodium acetate buffer with 10mM ETDA added (0.372g/100ml of buffer) for 10 minutes at pH 4.2 or 4.6. Wash in distilled water. Proceed as for the pH 9.4 method. NOTES Only the pH 4.2
Materials Purified Motor Protein, 20-80 µM Nucleotide Mix = 50 mM Mg・ATP gamma-32 P-ATP to give 5 000 - 10 000 cpm/nmol 10 mM HEPES, pH 7.2 1 mM EGTA 1 mM MgCl
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