- ￥31284.98
- Profoldin
- SDNA-1MG
- 2025年07月11日
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1 mg
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文献和实验Purification of Supercoiled Plasmid DNA
The topology of DNA affects a number of major biological processes (1 -4 ). For example, processive enzymes, such as the RNA and DNA polymerases, have the capability of generating both positive and negative DNA supercoils during the process
Alkali Denaturation of Supercoiled Plasmid DNA
实验步骤 1. To 20 ul of purified, concentrated DNA solution add 2 ul of freshly prepared NaOH/EDTA solution and incubate at 37 degrees for 30 minutes. 2. Neutralize the reaction by adding : 3 ul 3M NaOAc 5.2 and 7 ul H2
Purification of Supercoiled Plasmid
in compromised purity (1 ,2 ). The success of DNA vaccines in animal models and the initiation of human trials (3 ,4 ) has led to a need to increase the level of supercoiled plasmid purity as well as the methodology utilized to produce these plasmids at large
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