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文献和实验Functional Analysis of Mutant Mitochondrial DNA Polymerase Proteins Involved in Human Disease
DNA polymerase γ (pol γ) is the only DNA polymerase within the mitochondrion and is thus essential for replication and repair of mtDNA. POLG , the gene encoding the catalytic subunit of pol γ, is a major locus for a wide spectrum
The Polymerase Chain Reaction (PCR)
a means of selectively amplifying a particular segment of DNA. • The segment may represent a small part of a large and complex mixture of DNAs:e.g. a specific exon of a human gene. • It can be thought of as a molecular photocopier. A Molecular
several assays for detecting several kinds of DNA damage (strand breaks, internal crosslinking, DNA/protein crosslinks) and repair activity following exposure to genotoxic agents. The methods include single?cell electrophoresis (comet assay), filter eluting, K
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