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文献和实验Assays for Hepatitis B Virus DNA-and RNA-Dependent DNA Polymerase Activities
polymerase II, into unspliced, capped polyadenylated mRNA species from which viral proteins are transcribed. In addition, terminally redundant 3.5-kb RNA transcripts, which function as pregenomes, are produced and exported to the cytoplasm
Hepatitis C Virus RNA-Dependent RNA Polymerase (NS5B Polymerase)
studies of several recombinant HCV nonstructural proteins have been initiated, our knowledge of the HCV NS5B polymerase that encodes an RNA-dependent RNA polymerase (RDRP) is rudimentary (4 –8 ). RNA-dependent RNA polymerases represent a class of viral
Native Gel Electrophoresis to Study the Binding and Release of RNA Polymerase by 6S RNA
protein complexes, which is often called band shift, gel retardation, or electrophoretic mobility shift assays. Gel shift assays have been used to study a plethora of RNA–protein interactions in all organisms, but here we will use the 6S RNA:RNA polymerase
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