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文献和实验Steady State ATPase Assays Coupled Enzyme System
from Rabbit Muscle in 50% glycerol) Procedure 1. Assemble microtubules for assays. E.g., 58 µL of 50 µM MTs = 50 µL 5.8 mg/mL tubulin + 0.5 µL 100 mM Mg・GTP, incubate for 30-60 min at 37°C
Functional Assays on High‐Content Protein Microarrays
?231 © 2012 by John Wiley & Sons, Inc. Keywords: high content protein microarray; functional assays; protein?protein interaction; small?molecule interaction; kinase substrate profiling; ubiquitin ligase substrate profiling; protein
Identification of a Mutant Kinase/ATP Analog Pair
analog and substrate in an in vitro kinase reaction. The reaction is then analyzed by sodium dodecylsulfate-polyacrylamide gel electrophoresis (SDS-PAGE), transferred to nitrocellulose, and either stained with Ponceau S and counted by Cerenkov counting
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