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100 assays
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文献和实验simply due to their ease of lysis. Other bacteria (e.g. XL-1Blue) are fine. Before the assay, prewash the Jun beads in PBST. 2. If you are only doing a substrate (eg. c-Jun kinase assay) interaction then add the 1 ml of lysate to the aliquot of beads
Strategies to Optimize Protein Expression in E. coli
in Escherichia coli (E. coli ) is simple, fast, inexpensive, and robust, with the expressed protein comprising up to 50 percent of the total cellular protein. However, it also has disadvantages. For example, the rapidity of bacterial protein expression
SAPK/Jun kinase assays Preparation of cell lysate: 1. For cells in suspension, grow in DMEM with 10% FCS and then the day before you do the experiment spin the cells down (1 K for 5 min) and obtain a concentration of cells
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