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文献和实验Strategies to Optimize Protein Expression in E. coli
in Escherichia coli (E. coli ) is simple, fast, inexpensive, and robust, with the expressed protein comprising up to 50 percent of the total cellular protein. However, it also has disadvantages. For example, the rapidity of bacterial protein expression
Introduction to the EMSA (Gel Shift) Technique
on linear DNA fragments result in the characteristic retarded mobility in the gel. However, if circular DNA is used (e.g., minicircles of 200-400 bp), the protein:DNA complex may actually migrate faster than the free DNA. Gel shift assays are also good
Introduction to the EMSA (Gel Shift) Technique
on linear DNA fragments result in the characteristic retarded mobility in the gel. However, if circular DNA is used (e.g., minicircles of 200-400 bp), the protein:DNA complex may actually migrate faster than the free DNA. Gel shift assays are also good
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