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100 assays
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文献和实验Methods to Assay Inhibitors of DNA Gyrase and Topoisomerase IV Activities
on DNA supercoiling by S. pneumoniae gyrase, DNA decatenation by S. pneumoniae topo IV, and DNA cleavage by both enzymes. These approaches provide mechanistic insight on inhibitor action and allow identification of dual gyrase/topo IV targeting agents
DNA Fragmentation Assays for Apoptosis
. Then cool the gel to 50oC and add 25ul of ethium bromide before pouring it into the gel plate. Insert comb and let the gel polymerized. Stock IV: Hindi III Marker (50 Kb lamda DNA) 4ul of Hindi III Marker 16ul of Deionized Water 4ul of Loading Buffer
DNA Fragmentation Assays for Apoptosis Protocol
ul of ethium bromide before pouring it into the gel plate. Insert comb and let the gel polymerized.Stock IV: Hindi III Marker (50 Kb lamda DNA) 4ul of Hindi III Marker 16ul of Deionized Water 4ul of Loading BufferProtocol III: DNA Fragmentation Assay
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