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文献和实验Bacillus subtilis , Caulobacter crescentus and Mycobacterium tuberculosis . Because E. coli can be grown to high cell densities relative to eukaryotes, it is possible to generate sufficient DNA to label without using a PCR-based method. This method uses
High Level Expression of Recombinant Proteins in E. coli
of mg quantities Part I. Expression Systems Expression System Components Target Protein mRNA cDNA genomic DNA Expression Vector (plasmids) pET pQE pGEX Expression Strain (E. coli ) BL21(DE3) Rosetta(DE3
Strategies to Optimize Protein Expression in E. coli
in Escherichia coli (E. coli ) is simple, fast, inexpensive, and robust, with the expressed protein comprising up to 50 percent of the total cellular protein. However, it also has disadvantages. For example, the rapidity of bacterial protein expression
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