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文献和实验Strategies to Optimize Protein Expression in E. coli
in Escherichia coli (E. coli ) is simple, fast, inexpensive, and robust, with the expressed protein comprising up to 50 percent of the total cellular protein. However, it also has disadvantages. For example, the rapidity of bacterial protein expression
, A.R., Barnum, J.E., Harmon, L.S., DeLuca, J.G., and Nichols, W.W. 1994. Rapid DNA degradation in primary rat hepatocytes treated with diverse cytotoxic chemicals: Analysis by pulsed‐field gel electrophoresis and implications for alkaline elution assays
Protein Purification: Assays, Specific Activity, Initial Fractionation
in E. coli , or trying to isolate a protein from some mammalian tissue, you are typically starting with gram quantities of a complex mixture of protein, nucleic acids, polysaccharide, etc. from which you may have to extract milligram (or microgram
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