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文献和实验In Vitro Assessment of the Fitness of Resistant M. tuberculosis Bacteria by Competition Assay
Bacteria become resistant by a number of different mechanisms, and these include mutation in chromosomal genes (1 ), acquisition of plasmids (2 ), insertion of bacteriophage, transposon or insertion sequence DNA (3 -5 ), or gene mosaicism
were isolated from EHS using PBS/EDTA, and also cross-linked as cells growth in 2D. 2, Wash cells twice using ice cold PBS containing protease inhibitors (1mM phenylmethylsulfonyl fluoride (PMSF), 1microgram/ml aprotinin and 1microgram/ml pepstatin
ml: 93.84 g] Pbs: [for 1l: 8 g NaCl; 0.2 g KCl; 1.15 g Na2 HPO4 * 7H2 O; 0.2 g KH2 PO4 ] CHIP lysis buffer: 50 mM HEPES pH 7.5; 140 mM NaCl; 1 % Triton X100; 0.1 % NaDeoxycholate; protease inhibitors. [ for 500 ml: 25 ml 1 M HEPES pH
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