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文献和实验Assay for ß-galactosidase in E. Coli
in the cell only when an inducer (signal) is present in the cell (e.g. ß-galactosidase or the enzymes in the tryptophan biosynthesis pathway). This procedure is designed to induce and measure the level of ß-galactosidase in E. coli. Cells grown
Direct Kinase Assay Screening for Inhibitors of MAP Kinase
interest in cancer studies including ovarian cancer. Studies on MAP kinase activation and regulation will lead to an improved understanding of intracellular signal transduction and involvement of this enzyme in key signaling process. It is therefore important to know
Strategies to Optimize Protein Expression in E. coli
in Escherichia coli (E. coli ) is simple, fast, inexpensive, and robust, with the expressed protein comprising up to 50 percent of the total cellular protein. However, it also has disadvantages. For example, the rapidity of bacterial protein expression
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