E. coli GlmU Assay Kit Plus-50

ChIP-Chip E. coli

is the same for all species, there are some differences in handling cells. This protocol is developed and tested for E. coli . It should work the same way for other bacteria but that remains to be proven. Published protocols also exist for other bacterial species, including

ELECTROPORATING E. COLI

1.O/N (5ml E.coli in LB-->1ml into 200ml LB/37℃ 2.Grow 2-3H to A660 ~0.3-0.4 3.Chill cells 10'/ice Spin down 10',5K,4℃ 4.Wash 1X 200ml 10% glycerol (sterile)40℃; Spin down 10',5K,4℃ 5.Wash 1X 40 ml 10% glycerol--> transfer to S34 tubes & spin

Purification of TFIIA from E. coli

.A protein assay is done,then the two subunits are mixed together in equimolar amounts at 0.4 � 1 mg/ml (additional RB+ 7M urea is added to obtain correct concentration for renaturation).The two subunits are then renatured together during dialysis against RB