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文献和实验Methods to Assay Inhibitors of DNA Gyrase and Topoisomerase IV Activities
on DNA supercoiling by S. pneumoniae gyrase, DNA decatenation by S. pneumoniae topo IV, and DNA cleavage by both enzymes. These approaches provide mechanistic insight on inhibitor action and allow identification of dual gyrase/topo IV targeting agents
James Hardwick's angiotensin assay protocol
This specific procedure was developed to assay the activity of the Lck kinase expressed from a retroviral vector in rat fibroblasts.You can obviously use fewer or more cells,depending on your needs.Don't skimp too much on the amount of S.aureus bugs
A High-Throughput Assay for DNA Topoisomerases and Other Enzymes, Based on DNA Triplex Formation
We have developed a rapid, high-throughput assay for measuring the catalytic activity (DNA supercoiling or relaxation) of topoisomerase enzymes that is also capable of monitoring the activity of other enzymes that alter the topology of DNA
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