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- Profoldin
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- 2025年07月15日
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文献和实验Methods to Assay Inhibitors of DNA Gyrase and Topoisomerase IV Activities
on DNA supercoiling by S. pneumoniae gyrase, DNA decatenation by S. pneumoniae topo IV, and DNA cleavage by both enzymes. These approaches provide mechanistic insight on inhibitor action and allow identification of dual gyrase/topo IV targeting agents
Fine-Tuning Enzyme Activity Through Saturation Mutagenesis
Codon saturation is a powerful tool for analyzing protein structure–function relationships and fine-tuning enzyme activity. In this technique, one or more key amino acids are randomized by incorporating degenerate codon(s) into a gene
Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
of interest. The immunoprecipitated DNA can then be amplified using primers spanning the restriction enzyme site found to be involved in the 3C ligation with other partner(s) in each of the 6C clones. In early attempts of the 6C assay (Tiwari et al. 2008), screening
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