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- Profoldin
- D87G100KE
- 2025年07月10日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
999
- 规格：
100 assays
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文献和实验is the same for all species, there are some differences in handling cells. This protocol is developed and tested for E. coli . It should work the same way for other bacteria but that remains to be proven. Published protocols also exist for other bacterial species, including
Purification of TFIIA from E. coli
.Supernatant is discarded,pellets are washed in 100ml RB,and pelleted again.Supernatant is discarded and the pellets are resuspended in RB Buffer+ 7M urea+ 5mM DTT+ PIs.This is incubated on a platform rotator for 1 hour at 4 degrees to resolubilize the subunits
Purification of TFIIA from E. coli
Purification of TFIIA from E.coli Linda Warfield,Hahn Lab,2003 Volumes given are for 2L of cells for each subunit (Toa1 and Toa2). Toa1 and Toa2 subunits are expressed separately in cultures of OD0.6 for 2 hours with 0.4mM IPTG at 37 degrees
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