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文献和实验Steady State ATPase Assays Coupled Enzyme System
, in Tris-HCl. Avoid using phosphate buffers for ATPase assays. 2. The coupled enzyme ATPase assay is based on the conversion of phosphoenolpyruvate (PEP) to pyruvate by pyruvate kinase (PK) coupled to the conversion
In order to characterize the P-type ATPase from Synechocystis 6803 [Geisler (1993) et al. J. Mol. Biol. 234 , 1284] and to facilitate its purification, we expressed an N-terminal 6xHis-tagged version of the ATPase in an ATPase deficient E. coli
Assay for ß-galactosidase in E. Coli
in the cell only when an inducer (signal) is present in the cell (e.g. ß-galactosidase or the enzymes in the tryptophan biosynthesis pathway). This procedure is designed to induce and measure the level of ß-galactosidase in E. coli. Cells grown
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