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文献和实验Detection of Telomerase Enzyme Activity by TRAP Assay
The telomeric repeat amplification protocol (TRAP) assay, first published by Kim et al. in 1994 (1 ), is used to detect telomerase activity. The scope of the technique is far reaching and has implications for telomere, telomerase, aging
Histochemical staining of sea urchin embryos for alkaline phosphatase (AP) enzyme activity
Histochemical staining of sea urchin embryos for alkaline phosphatase (AP) enzyme activity 1. Obtain embryo samples, tube of AP substrate buffer and tube of phosphate buffered saline ( PBS ) for each group. Allow embryos
Assessment and Quantification of Telomerase Enzyme Activity
The enzyme telomerase is activated in 80–90% of all human malignancies and immortal cell lines, where it functions to maintain the integrity of chromosomal-end structures called telomeres. Telomerase enzyme activity can be detected
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