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文献和实验Drug Toxicity in E. coli Cells Expressing Human Topoisomerase I
for the major DNA-relaxing activity of the cell and is essential in an otherwise wild-type E. coli (3 ). E. coli and human topo I (htopoI) both catalyze the relaxation of negatively supercoiled DNA. However, they differ in the details of the reactions they catalyze
Transformation DNA fragments (or plasmid DNA) into competent E. coli
Transformation DNA fragments (or plasmid DNA) into competent E. coli * Caution: Use aerosol protecting tips if selection of transformed cells is not based on X-gal strategy. 1.Remove competent cells (E.coli DH5aTM from GIBCO BRL) from -70 ℃
Bacillus subtilis , Caulobacter crescentus and Mycobacterium tuberculosis . Because E. coli can be grown to high cell densities relative to eukaryotes, it is possible to generate sufficient DNA to label without using a PCR-based method. This method uses
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