E. coli DNA Topoisomerase I As

Drug Toxicity in E. coli Cells Expressing Human Topoisomerase I

for the major DNA-relaxing activity of the cell and is essential in an otherwise wild-type E. coli (3 ). E. coli and human topo I (htopoI) both catalyze the relaxation of negatively supercoiled DNA. However, they differ in the details of the reactions they catalyze

ChIP-Chip E. coli

Bacillus subtilis , Caulobacter crescentus and Mycobacterium tuberculosis . Because E. coli can be grown to high cell densities relative to eukaryotes, it is possible to generate sufficient DNA to label without using a PCR-based method. This method uses

E.Z.N.A.® Endo-free Plasmid Mini Kit I Spin Protocol

as above. This step ensures that residual protein contamination is removed and must be included for downstream application requiring high quality DNA. 12. Discard the flow-through liquid and wash the column by adding 700 u DNA Wash Buffer diluted with ethanol