S. pneumoniae DNA Helicase ATP

Steady State ATPase Assays Coupled Enzyme System

, in Tris-HCl. Avoid using phosphate buffers for ATPase assays. 2. The coupled enzyme ATPase assay is based on the conversion of phosphoenolpyruvate (PEP) to pyruvate by pyruvate kinase (PK) coupled to the conversion

ChIP assay: Chromatin immunoprecipitation assay was performed according to the protocol of ChIP assay kit

  1, For cells cultured in 2D, about 1-2X107 S1 cells were growth in 100mm dish, and were cross-linked by adding formaldehyde to final concentration of 1% and incubated in room temperature for 10 minutes. For cells growth in 3D, cells

MicroElute DNA Clean-Up Kit

Clean-up Spin Protocol For DNA fragments (30bp-100 bp)    1) Add 3 x volume of buffer P3, 3 x volume of isopropanol to the enzymatic reaction and mix throughly with pipetting or vortex. The maximum volume of the reaction can be processed