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文献和实验Protocol for intracytoplasmic staining of cytokines for FACS analysis
S-2149). 7) We suggest the following antibody conjugates (usually at 10mcg/ml or as manufacturers recommendations) to detect mouse cytokines, added in saponin buffer for 30 mins at 4 deg C. I have grouped them in compatible pairs for multicolour
Immunohistochemisty-Fluorescence Protocol
antibody (either biotin-donkey anti-goat IgG Fab2 diluted 1:700 or biotin-goat anti-mouse IgG1 or IgG2A or IgG2B diluted 1:500) in Wash Buffer-Saponin. Wash the cells twice in Wash Buffer-Saponin. Incubate the centrifuged cell pellet
Immunohistochemistry: Fluorescence Protocol 2.0
by centrifugation (400 x g for 5 minutes). Incubate the cells for 30 minutes at room temperature with 50 µL of a biotinylated secondary antibody (either biotin-donkey anti-goat IgG Fab2 diluted 1:700 or biotin-goat anti-mouse IgG1 or IgG2A or IgG2B diluted
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